- Objective magnification
- 40×
- Eyepiece magnification
- 10×
400×
Open with these values400×
Result: 400 ×Total magnification is the objective times the eyepiece: a 40× objective with a 10× eyepiece gives 400×. Some stands add a third factor — a tube lens of 1.25× or 1.5×; multiply that in yourself, this calculator uses the two named lenses. Magnification is not resolution.
Held fixed: Objective magnification 40.00 ×.
| Eyepiece magnification (×) | Result (×) |
|---|---|
| 2.50 | 100 |
| 5.00 | 200 |
| 7.50 | 300 |
| 10.00Your value | 400 |
| 12.50 | 500 |
| 15.00 | 600 |
| 17.50 | 700 |
| 20.00 | 800 |
400×
Open with these values1,000×
Open with these values40×
Open with these valuesTotal = objective × eyepiece
A compound microscope magnifies twice over. The objective forms an enlarged image inside the tube and the eyepiece enlarges that image again, so the two factors compound rather than add, and the total is their product. With the defaults, a 40× objective under a 10× eyepiece gives 400×, the working setting of almost every school microscope. Because the eyepiece is a fixed 10× on most stands, the entire range comes out of the turret: 4×, 10×, 40× and 100× against that eyepiece produce 40×, 100×, 400× and 1000×. The total is therefore a property of the current setting rather than of the instrument, and it changes with every click of the nosepiece. What the product does not record is which pair produced it. The objective decides what you are actually doing — finding the specimen, surveying tissue, working dry at high power or going into oil — so two pairings that reach the same total are not the same view. The limitation sits in the second field. This page multiplies exactly the two lenses you name, and some stands carry a third element between them, an intermediate or tube lens of 1.25× or 1.5×, printed on the body. There is deliberately no field for it: a default of 1 would be dead weight on the great majority of microscopes, and a silently built-in 1.25 would be plainly wrong on every stand without such a lens. Check your own and multiply it in.
Magnification only makes the image larger; resolution decides how much detail is there at all. Past a point you get a bigger blur — empty magnification — and no new detail.
Some stands carry an intermediate lens of 1.25× or 1.5× between objective and eyepiece, printed on the stand. This calculator takes the two lenses you name, so multiply that factor in yourself.
It is a ratio, written with a multiplication sign as in 400×. At 400× the specimen looks 400 times larger than to the unaided eye.
A 1000× oil-immersion view shows more detail than 400×.
It shows a larger image, not automatically a sharper one — resolution decides the detail. Beyond that limit you only magnify the blur.
Objective and eyepiece are added together.
They are multiplied: a 40× objective with a 10× eyepiece gives 400×.
The 400× I get here is what my stand really delivers.
Only when there is no tube lens. An intermediate lens of 1.25× or 1.5× multiplies on top, and this calculator uses just the two named lenses.
| Objective, eyepiece | What it is for | Total |
|---|---|---|
| 4, 10 | Scanning, finding the specimen | 40 |
| 10, 10 | Low power, tissue overview | 100 |
| 40, 10 | High dry, cells and nuclei | 400 |
| 60, 15 | High dry with a wide-field eyepiece | 900 |
| 100, 10 | Oil immersion, bacteria | 1000 |
Multiply the objective magnification by the eyepiece magnification. On a compound light microscope with a 40× objective and a 10× eyepiece the total is 400×.
Then the total is objective × eyepiece × tube factor, and that factor is usually 1.25× or 1.5× printed on the stand. This calculator takes the two lenses you name, so multiply the tube factor in afterwards.
Most school and lab microscopes pair a 10× eyepiece with 4×, 10×, 40× and 100× objectives, giving 40×, 100×, 400× and 1000×. The 100× oil-immersion objective is the strongest on a typical light microscope.
No. Magnification only makes the image larger, while resolution decides how much detail is actually there. Past a point you get a bigger blur — empty magnification — and no new detail.
None. Magnification is a ratio and is written with a multiplication sign, as in 400×, meaning the specimen looks 400 times larger than to the unaided eye.
Information, not professional advice.
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